LOCUS pVenus-C1-Cdc20 (1534) 6207 bp DNA circular SYN 26-AUG-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6207)
AUTHORS Nilsson J, Yekezare M, Minshull J, Pines J
TITLE The APC/C maintains the spindle assembly checkpoint by targeting
Cdc20 for destruction.
JOURNAL Nat Cell Biol. 2008 Dec . 10(12):1411-20.
PUBMED 18997788
REFERENCE 2 (bases 1 to 6207)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell
Biol. 2008 Dec . 10(12):1411-20."
FEATURES Location/Qualifiers
source 1..6207
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 547..556
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 553..1269
/codon_start=1
/product="yellow fluorescent protein (YFP) with fast and
efficient maturation (Nagai et al., 2002)"
/label=Venus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(598..619)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(859..878)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1206..1227
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2935..3056
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2972..2991)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3026..3045
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3063..3518)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3200..3221)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3412..3431
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3545..3649
/gene="bla"
/label=AmpR promoter
promoter 3651..4008
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 3651..3842
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(3674..3694)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3859..3994
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4043..4837
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 4043..4837
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4097..4116)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4707..4726
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5025..5044)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5069..5116
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5445..6033
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5934..5953
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
661 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
721 ccctggccca ccctcgtgac cactctgggc tacggcctac agtgcttcgc ccgctacccc
781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
1021 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1141 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctatggc acagttcgcg
1321 ttcgagagtg acctgcactc gctgcttcag ctggatgcac ccatccccaa tgcaccccct
1381 gcgcgctggc agcgcaaagc caaggaagcc gcaggcccgg ccccctcacc catgcgggcc
1441 gccaaccgat cccacagcgc cggcaggact ccgggccgaa ctcctggcaa atccagttcc
1501 aaggttcaga ccactcctag caaacctggc ggtgaccgct atatccccca tcgcagtgct
1561 gcccagatgg aggtggccag cttcctcctg agcaaggaga accagcctga aaacagccag
1621 acgcccacca agaaggaaca tcagaaagcc tgggctttga acctgaacgg ttttgatgta
1681 gaggaagcca agatccttcg gctcagtgga aaaccacaaa atgcgccaga gggttatcag
1741 aacagactga aagtactcta cagccaaaag gccactcctg gctccagccg gaagacctgc
1801 cgttacattc cttccctgcc agaccgtatc ctggatgcgc ctgaaatccg aaatgactat
1861 tacctgaacc ttgtggattg gagttctggg aatgtactgg ccgtggcact ggacaacagt
1921 gtgtacctgt ggagtgcaag ctctggtgac atcctgcagc ttttgcaaat ggagcagcct
1981 ggggaatata tatcctctgt ggcctggatc aaagagggca actacttggc tgtgggcacc
2041 agcagtgctg aggtgcagct atgggatgtg cagcagcaga aacggcttcg aaatatgacc
2101 agtcactctg cccgagtggg ctccctaagc tggaacagct atatcctgtc cagtggttca
2161 cgttctggcc acatccacca ccatgatgtt cgggtagcag aacaccatgt ggccacactg
2221 agtggccaca gccaggaagt gtgtgggctg cgctgggccc cagatggacg acatttggcc
2281 agtggtggta atgataactt ggtcaatgtg tggcctagtg ctcctggaga gggtggctgg
2341 gttcctctgc agacattcac ccagcatcaa ggggctgtca aggccgtagc atggtgtccc
2401 tggcagtcca atgtcctggc aacaggaggg ggcaccagtg atcgacacat tcgcatctgg
2461 aatgtgtgct ctggggcctg tctgagtgcc gtggatgccc attcccaggt gtgctccatc
2521 ctctggtctc cccattacaa ggagctcatc tcaggccatg gctttgcaca gaaccagcta
2581 gttatttgga agtacccaac catggccaag gtggctgaac tcaaaggtca cacatcccgg
2641 gtcctgagtc tgaccatgag cccagatggg gccacagtgg catccgcagc agcagatgag
2701 accctgaggc tatggcgctg ttttgagttg gaccctgcgc ggcggcggga gcgggagaag
2761 gccagtgcag ccaaaagcag cctcatccac caaggcatcc gctgaggatc caccggatct
2821 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
2881 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
2941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
3001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
3061 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
3121 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
3181 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
3241 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
3301 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
3361 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
3421 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
3481 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
3541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
3601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
3661 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
3781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
3841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
3901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
3961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
4021 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
4081 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
4141 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
4201 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
4261 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
4321 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
4381 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
4441 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
4501 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
4561 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
4621 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
4681 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
4741 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
4801 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
4861 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
4921 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
4981 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
5041 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
5101 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
5161 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
5221 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
5281 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
5341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
5401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
5461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
5521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
5581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtatca
5641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
5701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
5761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
5821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
5881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
5941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
6001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
6061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
6121 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
6181 agttcatagc ccatatatgg agttccg
//
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